Fullerene Nanomaterials Inhibit the Allergic Response
John J. Ryan, Henry R. Bateman, Alex Stover, Greg Gomez, Sarah K. Norton, Wei Zhao, Lawrence B. Schwartz, Robert Lenk and Christopher L. Kepley
J Immunol July 1, 2007, 179 (1) 665-672; DOI: https://doi.org/10.4049/jimmunol.179.1.665
Protein microarray demonstrated that inhibition of mediator release involves profound reductions in the activation of signaling molecules involved in mediator release and oxidative stress. Follow-up studies demonstrated that the tyrosine phosphorylation of Syk was dramatically inhibited in Ag-challenged cells first incubated with fullerenes. In addition, fullerene preincubation significantly inhibited IgE-induced elevation in cytoplasmic reactive oxygen species levels. Furthermore, fullerenes prevented the in vivo release of histamine and drop in core body temperature in vivo using a MC-dependent model of anaphylaxis. These findings identify a new biological function for fullerenes and may represent a novel way to control MC-dependent diseases including asthma, inflammatory arthritis, heart disease, and multiple sclerosis.