The antimicrobial activities of fullerene C60 and its derivatives against 6 kinds of bacteria and 2 kinds of fungi were evaluated. The tested samples were water-soluble fullerenes (polyvinylpyrrolidone (PVP)/C60, γ-cyclodextrin (γ-CD)/C60, and nano-C60) and 3 types of fullerenols (C60(OH)12, C60(OH)36·8H2O, and C60(OH)44·8H2O).